The molecular biology of UDP-glucuronyltransferases.

نویسندگان

  • B Burchell
  • M R Jackson
  • R B Coarser
  • M W Coughtrie
  • D Harding
  • S H Shepherd
  • S M Wilson
چکیده

Beale. D.. Ketterer. B., Carne, T., Meyer, D. & Taylor, J . B. (1982) Eur. J. Biochem. 126. 459 463 Beale. D.. Meyer, D., Taylor, J. B. & Ketterer. B. (1983) Eur. J. Biochem. 137. 125 129 Ding. G. J.-F.. Lu. A. Y. H. & Pickett, C. B. (1985) J. B i d . Chem. 260. 13268 13271 Jakoby. W. B. & Habig. W. H. (1980) in Enzymutic Basis qf DeroxiCU/ i (Jn (Jakoby. W. B., ed.), vol. 2. pp. 63--94, Academic Press, New York Ketterer. B.. Meyer, D. J., Coles, B., Taylor, J . B. & Pemble, S. E. ( 1986) in Antimu/agenesis and Anticarcinogenesis: Mechanisms (Shankel. D. M.. Hartman, P., Kada, T. & Hollaender, A,, eds.), pp. 103 126, Plenum Press, New York Lai. H.-C. J., Li. N.-Q., Weiss, M. J., Reddy. C. C. & Tu, C.-P. D . (1984) J . BifJ/. Chem. 259, 553&5542 Lai. H.-C. J.. Grove. G. & Tu, C.-P. D. (1986) Nucleic Acids Res. 14. 6101 6114 Mannervik, B. (1985) Adv. Enzymol. Relur. Areus Mol . Biol. 57,

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Diazobenzenesulphonate selectively abolishes stimulation of glucuronidation by UDP-N-acetylglucosamine.

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The molecular weights of UDP-glucuronyltransferase determined with radiation-inactivation analysis. A molecular model of bilirubin UDP-glucuronyltransferase.

The molecular masses of the UDP-glucuronyltransferases were determined by means of radiation-inactivation analysis of sodium dodecyl sulfate-treated lyophilized rat liver microsomal preparations using a calibrated 60Co source. Bilirubin glucuronidation with formation of bilirubin monoglucuronide is catalyzed by a 41,500-Da enzyme; conversion of bilirubin mono- to diglucuronide is catalyzed by a...

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Studies on the genetic linkage of bilirubin and androsterone UDP-glucuronyltransferases by cross-breeding of two mutant rat strains.

Gunn rats, which have defects in bilirubin and 4-nitrophenol UDP-glucuronyltransferases (GT), were crossed with LA Wistar rats with a defect in androsterone GT. The F1 hybrids showed normal GT activities towards androsterone, bilirubin and 4-nitrophenol, demonstrating that Gunn and LA ('low activity') Wistar rats inherit a homozygous dominant trait for androsterone GT and bilirubin GT respectiv...

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Identification and characterization of three Drosophila melanogaster glucuronyltransferases responsible for the synthesis of the conserved glycosaminoglycan-protein linkage region of proteoglycans. Two novel homologs exhibit broad specificity toward oligosaccharides from proteoglycans, glycoproteins, and glycosphingolipids.

The Drosophila melanogaster genome contains three putative glucuronyltransferases homologous to human GlcAT-I and GlcAT-P. These enzymes are predicted to be beta1,3-glucuronyltransferases involved in the synthesis of the glycosaminoglycan (GAG)-protein linkage region of proteoglycans and the HNK-1 carbohydrate epitope of glycoproteins, respectively. The genes encode active enzymes, which we hav...

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In situ structural analysis of microsomal UDP-glucuronyltransferases by radiation inactivation.

The structure of the UDP-glucuronyltransferases in microsomes from guinea pig and rat liver was examined in situ by radiation inactivation analysis. The p-nitrophenol conjugating activity of guinea pig microsomes increased at lower doses of radiation; at higher doses (greater than or equal to 36 megarads), activity showed a first order decline yielding a target size of 71 +/- 9 kDa. Treating mi...

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عنوان ژورنال:
  • Biochemical Society transactions

دوره 15 4  شماره 

صفحات  -

تاریخ انتشار 1987